AALL1931: Erwinia asparaginase in ALL

At the 2022 American Society of Clinical Oncology (ASCO) Annual Meeting, Maese¹ presented new data on behalf of the Children’s Oncology Group on the AALL1931 study (NCT04145531), which investigated a recombinant Erwinia chrysanthemi asparaginase, JZP458, in patients with acute lymphoblastic leukemia (ALL) or lymphoblastic lymphoma (LBL). Similar to other asparaginases used in the treatment of ALL and LBL, JZP458 induces apoptosis in cancer cells.² A unique benefit of JZP458 is that it can be used in patients who are hypersensitive to E.coli products.¹ JZP458 was approved by the FDA in 2021 at a dose of 25 mg/m² for the treatment of ALL and LBL in patients ≥1 month old.^1,3

Study design¹

• Part A of this ongoing phase II/III trial enrolled 167 patients with ALL or LBL who experienced Grade ≥3 allergic reactions to E.coli-derived asparaginase. The study was split into two parts:
  • Part A involved administering JZP458 intramuscularly.
  • Part B involved administering JZP458 intravenously.
• Both part A and part B involved patients receiving six doses of JZP458 on a Monday–Wednesday–Friday (MWF) schedule.
• Maese presented results from part A of the study. Results from part B of the study are expected later this year.
• Part A consisted of three patient cohorts:
  • Cohort 1a received JZP458 at a dose of 25 mg/m² (MWF).
  • Cohort 1b received JZP458 at a dose of 37.5 mg/m² (MWF).
  • Cohort 1c received differential dosing of 25 mg/m² on Monday and Wednesday, and 50 mg/m² on Friday.

• The safety follow-up was at 30 days.

• The primary endpoints included safety and tolerability of the drug, with efficacy measured by the proportion of patients achieving a nadir serum asparaginase activity assessment (NSAA) level of ≥0.1 IU/mL in the last 72 hours of the first course of JZP458. A population pharmacokinetics (PopPK) model was developed based on SAA data to allow better dosing decisions and simulate efficacy in a larger population (2,000 patients).

Results¹

A total of 167 patients were dosed in part A of this trial; patient characteristics are shown below in Table 1.

 

Table 1. Patient characteristics*

B-ALL, B-cell acute lymphoblastic leukemia; B-LBL, B-cell lymphoblastic lymphoma; T-ALL, T-cell acute lymphoblastic leukemia; T-LBL, T-cell lymphoblastic lymphoma. *Adapted from Maese.1
Characteristics	Cohort 1a (n = 33)	Cohort 1b (n = 83)	Cohort 1c (n = 51)
Median age, years (range)	11 (1–24)	8 (1–20)	12 (3–25)
<6, n (%)	9 (27)	24 (29)	11 (22)
6 to <12, n (%)	9 (27)	34 (41)	14 (27)
12 to <18, n (%)	7 (21)	20 (24)	18 (35)
≥18, n (%)	8 (24)	5 (6)	8 (16)
Male, n (%)	17 (52)	55 (66)	31 (61)
Primary disease, n (%)
B-ALL	27 (82)	60 (72)	37 (73)
T-ALL	4 (12)	13 (16)	9 (18)
B-LBL	0	0	1 (2)
T-LBL	2 (6)	10 (12)	4 (8)
Median time (range) since last asparaginase dose received, days	9 (2–116)	10 (2–158)	10 (2–133)
Eligibility criteria met, n (%)
Grade ≥3 allergic reaction to an E.coli-derived asparaginase	27 (82)	75 (90)	44 (86)
Silent inactivation	3 (9)	3 (4)	1 (2)
Allergic reaction with inactivation	3 (9)	5 (6)	6 (12)

Efficacy outcomes

• The proportion of patients reaching the primary endpoint of an NSAA level of ≥0.1 IU/mL in the last 72 hours (first treatment course) is presented in Figure 1.
• Mean SAA levels (first treatment course) in the last 72 hours were
  • 0.16 IU/mL for cohort 1a;
  • 0.33 IU/mL for cohort 1b; and
  • 0.47 IU/mL for cohort 1c.

NSAA, nadir serum asparaginase activity.
*Adapted from Maese.¹

• The model simulated that 92% of patients in Cohort 1c would reach ≥0.1 IU/mL in the last 72 hours of the first treatment course; the observed proportion was 90%.
• Following intramuscular administration, the PopPK model that best described the PK of JZP458 was a 1-compartment intramuscular model with mixed-order absorption and linear elimination.
• The geometric mean half-life for JZP458 was estimated at 19.1 hours following intramuscular administration.

Safety analysis

• Overall, 6%, 17%, and 10% of treatment-related adverse events lead to drug discontinuation in cohorts 1a, 1b, and 1c, respectively.
• The primary reasons for discontinuation included pancreatitis, drug hypersensitivity, and anaphylactic reaction.
• There were no treatment-related adverse events leading to death.

Conclusion

The findings from part A of the AALL1931 trial demonstrate the efficacy of intramuscular JZP458 at 25/25/50 mg/m² in patients with ALL or LBL. The safety profile shows the treatment is well tolerated, with a similar safety profile to other asparaginases. Data from part B of this study, expected later this year, will provide more clarification on the potential of using JZP458 intravenously, and the best route of administration of the drug.