All content on this site is intended for healthcare professionals only. By acknowledging this message and accessing the information on this website you are confirming that you are a Healthcare Professional. If you are a patient or carer, please visit Know ALL.

The ALL Hub uses cookies on this website. They help us give you the best online experience. By continuing to use our website without changing your cookie settings, you agree to our use of cookies in accordance with our updated Cookie Policy

Introducing

Now you can personalise
your ALL Hub experience!

Bookmark content to read later

Select your specific areas of interest

View content recommended for you

Find out more
  TRANSLATE

The ALL Hub website uses a third-party service provided by Google that dynamically translates web content. Translations are machine generated, so may not be an exact or complete translation, and the ALL Hub cannot guarantee the accuracy of translated content. The ALL Hub and its employees will not be liable for any direct, indirect, or consequential damages (even if foreseeable) resulting from use of the Google Translate feature. For further support with Google Translate, visit Google Translate Help.

Steering CommitteeAbout UsNewsletterContact
LOADING
You're logged in! Click here any time to manage your account or log out.
LOADING
You're logged in! Click here any time to manage your account or log out.

The ALL Hub is an independent medical education platform, sponsored by Jazz Pharmaceuticals, Amgen, and Pfizer. The funders are allowed no direct influence on our content. The levels of sponsorship listed are reflective of the amount of funding given. View funders.

2024-11-21T17:17:58.000Z

High DNA input and next-generation sequencing peripheral blood MRD detection in patients with BCP-ALL

Nov 21, 2024
Share:
Learning objective: After reading this article, learners will be able to cite a new clinical development in acute lymphoblastic leukemia.

Bookmark this article

A retrospective analysis selected paired BM-PB samples from 69 patients with BCP-ALL where MRD was detected in the BM by conventional RT-qPCR but not in the PB.1 Samples were reanalyzed with heightened sensitivity using increased DNA input and amplicon-based NGS. Results from this analysis were published in the British Journal of Haematology by Bendig et al.1

Key learnings
In PB samples using high DNA input RT-qPCR, standard DNA input NGS, and high DNA input NGS, MRD was detected in 32%, 23%, and 48% of samples, respectively. 
Considering all possible IGH markers improved NGS MRD detection in 52% of PB samples. 
Despite improved detection in PB, MRD levels remained consistently low in PB compared with BM, indicating that PB monitoring alone may not fully replace BM analysis and relying on PB alone could delay early relapse detection.  
Results from this analysis suggest that analysis of PB samples with high DNA input and NGS could be considered when BM samples are unavailable in patients with BCP-ALL.  

Abbreviations: BCP-ALL, B-cell precursor acute lymphoblastic leukemia; BM, bone marrow; IGH, immunoglobulin heavy chain; MRD, measurable residual disease; NGS, next-generation sequencing; PB, peripheral blood; RT-qPCR, real-time quantitative polymerase chain reaction.

  1. Bendig S, Bufe S, Kotrova M, et al. Next-generation sequencing and high DNA input identify previously missed measurable residual disease in peripheral blood of B-cell precursor acute lymphoblastic leukaemia. Br J Haematol. 2024. Online ahead of print. DOI: 10.1111/bjh.19834

More about...

Your opinion matters

HCPs, what is your preferred format for educational content on the ALL Hub?
5 votes - 57 days left ...

Newsletter

Subscribe to get the best content related to ALL delivered to your inbox